Precision-cut liver slices: a versatile tool to advance liver research
نویسندگان
چکیده
منابع مشابه
Precision cut tissue slices of the liver as morphological tool for investigation of apoptosis.
BACKGROUND Apoptosis, programmed cell death, is involved in a broad range of pathological processes. Dysregulation of apoptosis plays a key role in the pathogenesis of hepatitis, toxic liver disease and also liver tumor development. For the study of apoptosis in liver diseases, different in vivo models and different in vitro approaches have been developed. They include cell culture models based...
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Chronic liver injury of various etiologies can cause liver fibrosis, which is characterized by the progressive accumulation of connective tissue in the liver. As no effective treatment for liver fibrosis is available yet, extensive research is ongoing to further study the mechanisms underlying the development of disease- or toxicity-induced liver fibrosis and to identify potential pro- or anti-...
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Alcohol abuse results in liver injury, but investigations into the mechanism(s) for this injury have been hampered by the lack of appropriate in vitro culture models in which to conduct in depth and specific studies. In order to overcome these shortcomings, we have developed the use of precision-cut liver slices (PCLS) as an in vitro culture model in which to investigate how ethanol causes alco...
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Introduction Hepatic macrophages have the capacity to secrete a tremendous array of molecules, which can be divided into 3 categories – cytokines (TNF-alpha), lipid mediators (prostaglandins PGE2) and reactive intermediates (NO·) – in response to stimulus, such as lipopolysaccharides (LPS) [1,2]. Such mediators are capable to modulate both the metabolism and the integrity of hepatocytes in vitr...
متن کاملDNA adduct formation in precision-cut rat liver and lung slices exposed to benzo[a]pyrene.
Chemical-DNA adducts provide an integrated measure of exposure, absorption, bioactivation, detoxification, and DNA repair following exposure to a genotoxic agent. Benzo[a]pyrene (BaP), a prototypical polycyclic aromatic hydrocarbon (PAH), can be bioactivated by cytochrome P-450s (CYPs) and epoxide hydrolase to genotoxic metabolites which form covalent adducts with DNA. In this study, we utilize...
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ژورنال
عنوان ژورنال: Hepatology International
سال: 2018
ISSN: 1936-0533,1936-0541
DOI: 10.1007/s12072-018-9913-7